Eun-Soo CHO, Jeong-Hwan KIM, Woo-Dam EONG, Yeon-Hee KIM, Soo-Wan NAM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129319
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원문정보
초록
영어
Agar is a structural polysaccharide found in cell walls of red algae and is generally considered to be a mixture of agarose and agaropectin. Enzymatic hydrolysis of sulfate groups in agaropectin or agar simplifies the production process of high-quality or low sulfate-content agarose. In this work, arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora was cell-surface displayed in S. cerevisiae and applied to desulfatate agar. The arylsulfatase gene was fused in frame with GAL1 promoter and AGA2 gene. To prepare the desulfatated agar, the commercial agar was treated with arylsulfatase displayed S. cerevisiae cell. The sulfate content in agar was decreased to 0.2% as a yeast biocatalyst concentration-dependent manner. Electrophoresis of λDNA HindⅢ marker and 1 kb DNA ladder with the arylsulfatase treated agar and commercial agarose showed the similar pattern of DNA migration and resolution. In addition, the gel strength of arylsulfatase treated agar and commercial agarose were compared.
Eun-Soo CHO [ Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Korea. ]
Woo-Dam EONG [ Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea. ]
Jeong-Hwan KIM [ Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Korea. ]
Yeon-Hee KIM [ Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea. ]
Soo-Wan NAM [ Department of Biotechnology and Bioengineering/Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동