Earticle

초록

영어

Bacilli are widely used as functional probiotics, feed and food additives. In this work, Bacillus sp. LS 1-2, was isolated from Korean transitional soybean paste foods and had similar 16S rRNA of Bacillus sp. of about 98%. LS 1-2 was cultured in citrus-processing-waste containing 5% solid as a culture medium and produced an antimicrobial agent against both of the gram-positives and negatives at adequate culture conditions (pH, Temperature, Culture period and etc). An antimicrobial agent produced by LS 1-2 was purified by alcohol precipitation, C-18 cartridge, YM-10, silica gel open column, prep TLC and prep HPLC. Antimicrobial activity was detected in the fermentation supernatant of LS 1-2. The active substance had more activity through the each purification step. Molecular weight of the antimicrobial agent was lower 10,000Da and it was a hydrophilic compound. The active substance was not sensitive to lipase, proteinase K, amylase, catalase but was affected by pronase E. So this antimicrobial agent was expected as a bacteriocin-like-substance. In order to evaluate its commercial value, the minimum inhibitory concentration of purified antimicrobial agent from Bacillus sp. LS 1-2 was compared with commercialized antimicrobial agents (Colistin, Chlortetracycline, Ampicillin) against Staphylococcus aureus and Escherichia coli O-157.

키워드

저자

• Jihyuk YU [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
• SunA YOON [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
• Jimin UM [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
• Dung Hoang NGUYEN [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
• Eun-Ki KIM [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576