In our previous study, recombinant E.coli harboring flavincontainingmonooxygenase(FMO) gene from Methylophaga aminisulfidivorans MPT produced indigo (853mg/L) and indirubin (≤5mg/L) in tryptophan medium (2g tryptophan, 10g NaCl, 5g yeast extract per liter). The goal of this study is to characterize the FMO and investigate the optimum culture conditions to enhance the amount of indirubin produced. The optimal pH andt emperature for FMO enzyme activity was found to be 8.5 and 45℃, respectively. The enzyme showed high affinity with trimethylamine (TMA). Vmax and Km values for trimethylamin were 19.3 nmol/min/mg protein and 23 μM, respectively. The optimum culture conditions determined by response surface method were as follows: 1g/L tryptophan, 5g/L yeast extract, 3mM cysteine, pH8.0 and temperature at 35℃. Under this condition, 15 times higher amount of indirubin (75.1mg/L) was produced.
키워드
Indirubinflavin-containing monooxygenaseindigo
저자
Seong Eun Bang [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Korea. ]
Gui Hwan Han [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Korea. ]
Si Wouk Kim [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동