Earticle

초록

영어

Nattokinase is a potent fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. The enzymes were discovered from various sources, such as Japanese natto, Korean Chungkookjang soy sauce, and Chinese douchi. Intravenous fibrinolytic agents such as urokinase and tissue plasminogen activator have been widely used in clinical practice for thrombolytic therapy. Microbial fibrinolytic enzymes have now attracted much more attention than typical thrombolytic agents because of the expensive prices and the undesirable side effects of the latter.In this study, Bacillus subtilis was cultivated to high cell density for nattokinase production. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on cell growth and nattokinase production. Among complex media, the highest cell growth (optical density monitored at 600 nm) and nattokinase activity were obtained with peptone supplementation. Amino acid analysis of the three complex media showed that glycine was richest in peptone. In fed-batch culture of B. subtilis, optimum ratio of peptone to glucose in feeding solution was 3:1. At this condition, cell concentration and nattokinase activity were 75 g/L and 15,000 unit/ml, respectively. By supplementing 2 g/L glycine in peptone-containing media, nattokinase activities increased compared without glycine supplementation

키워드

저자

• Eun-Yeong KWON [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]
• Beom Soo KIM [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576