Earticle

초록

영어

Mechanical parameters for mass production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica HS-2001 were optimized in pilot-scale bioreactors. The production of PS-7 and its conversion rate from 20.0 g/L glucose was 6.57 g/L and 33.0%. However, the maximal yield of total utilization (Yp/s + Yx/s) was 0.45 when the carbon source was 20.0 g/L sucrose. Higher inoculum size decreased the lag phase of cells and led to enhanced cell growth. Based on productivity and economic aspect, the optimal inoculum size for the production of PS-7 by B. ndica HS-2001 was decided to be 5.0% (v/v). The optimal agitation speed and aeration rate for cell growth of B. indica HS-2001 in a 100 L bioreactor were 300 rpm and 1.5 vvm, whereas those for the production of PS-7 were 250 rpm and 1.0 vvm. The optimal inner pressure for cell growth of B. indica HS-2001 in a 100 L bioreactor was 0.2 kgf/cm2, whereas that for the production of PS-7 was 0.4 kgf/cm2. The production of PS-7 by B. indica HS-2001 from 30.0 g/L sucrose with an optimized inner pressure of 0.4 kgf/cm2 was 9.02 g/L, which was 1.25 times higher than that without inner pressure in a 100 L bioreactor.

키워드

저자

• Eun-Jung LEE [ Dept. of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714, the Republic of Korea. ]
• Sang-Un LEE [ Dept. of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714, the Republic of Korea. ]
• Wa GAO [ Dept. of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714, the Republic of Korea. ]
• Jin-Woo LEE [ Dept. of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714, the Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576