Improved carotenoid yield by effective encystment as a result of combination of environmental stresses and antioxidant in the green unicellular alga, Haematococcus pluvialis
The induction stage of the unicellular alga, Haematococcus pluvialis, is constituted by two parts, encystment which is a morphological change from vegetative green cell to the cyst, and carotenogenesis that is to progress accumulating the economically valuable carotenoid, astaxanthin. And an effective induction requires the ROS (reactive oxygen species) generated by drastic environmental stresses like high light, high C/N ratio and high temperature. Therefore the microbe should be put on the pressure of transformation to encystment in the hard conditions. and such an intensive stresses lead to make the cell partially lethal by the burst of the oxidative damage so that a loss of the cell mass and the yield of astaxanthin. here we applied to combine an environmental stress (salt, sodium chloride) with an antioxidant (ascorbic acid) for the effective encystment to decrease the bio-factory loss caused by such an deadly damage, not for the case of carotenogenesis. The optimal condition to improve the carotenoid concentration was discovered at the 0.2%(w/v) NaCl with 0.1%(w/v) Ascorbic acid, cell mass 32% and total carotenoid concentration 23% enhanced respectively.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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