Leuconostoc citreum is the major lactic acid bacteria growing at an initial phase of kimchi fermentation. It grows fast at both aerobic and anaerobic conditions producing CO2, mannitol and dextran. In this study, high cell density culture of L. citreum was conducted in a fermentor equipped with an internal ceramic filtration system. The filter material was porous ceramic with pore size of 0.1 μm. The filter module consisted of 20 vertical cylindrical filter rods with inner diameter, outer diameter, and height of 6, 8, and 65 mm, respectively, and an upper frame of stainless steel. The total surface area of the filter module was ca. 330 cm2. The filter module was installed inside a 2.5-L jar fermentor. After 6 h of batch mode, filtration operation was started by feeding medium containing glucose, fructose, and yeast extract and at the same time by removing broth through the filter module. By using the internal filtration system, optical density at 660 nm increased to 70, which was 10 times higher than that in batch culture. The main co-products were mannitol and D-lactic acid. Mannitol and D-lactic acid concentrations increased to ca. 80 g/L and 40 g/L, respectively, by feeding fresh medium.
키워드
High cell density cultureLeuconostoc citreumMannitolD-lactic acid
저자
Ik-Kyoung SUNG [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]
Beom Soo KIM [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동