Earticle

초록

영어

To elucidate the function of an unknown regulator in Streptomyces, differences in phenotype and antibiotic production have been compared between a deletion mutant and wild type strain. However, these differences are easily hidden by complex media. Thus, we developed a multi-well method containing different nutrients along with bromothymol blue to determine the specific media conditions which can reveal such differences easily. By comparing the growth of wild type and mutant strains on the screened nutrient conditions, we were able to measure growth, organic acid production, and antibiotic production to elucidate the function of unknown regulator. As a result, a member of the MarR-like regulator family, SCO5405 (AbsC), was newly characterized. Deletion of SCO5405 increased the pH of the culture broth due to decreased production of organic acids such as pyruvate and α-ketoglutarate and increased extracellular ACT production in minimal media containing glucose and alanine.
Therefore, this method could be a high-throughput method for the characterization of unknown regulators.

키워드

저자

• Eunjung SONG [ Institute of Molecular Biology, School of Chemical and Biological Engineering, Seoul National University, Seoul. ]
• Yung-Hun YANG [ Department of Microbial Engineering, Konkuk University, Seoul. ]
• Bo-Rahm LEE [ Institute of Molecular Biology, School of Chemical and Biological Engineering, Seoul National University, Seoul. ]
• Eun-jung KIM [ Institute of Molecular Biology, School of Chemical and Biological Engineering, Seoul National University, Seoul. ]
• Ji-Nu KIM [ Institute of Molecular Biology, School of Chemical and Biological Engineering, Seoul National University, Seoul. ]
• Byung-Gee KIM [ Institute of Molecular Biology, School of Chemical and Biological Engineering, Seoul National University, Seoul. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576