Earticle

초록

영어

Cellulase have many industrial applications. Trichoderma reesei Rut C-30 produced high leveles of endo-β-1,4 glucanase and exo-β-1,4-glucanase. Cellulase production of Trichoderma reesei Rut C-30 with various initial concentrations of EM in medium producing cellulase was investigated. The conpositions of culture medium and condition are : Avicel 10g/l, Wheat Bran 50g/l, (NH4)2SO4 2.0g/l, Proteose Peptone 3.0g/l, Yeast Extract 0.5g/l, KH2PO4 4.0g/l, MgSO4·7H2O 0.3g/l, CaCl2·2H2O 0.3g/l, Tween-80 0.2ml/l, and 28℃ with 200rpm in shaking incubator for 6 days. Carboxymethyl cellulose(CMC) and filter paper(FP) activities were determined according to the method of the International Union of Pure and Applied Chemistry(IUPAC). The amount of reducing sugar liberated was determined by the dinitrosalicylic acid(DNS) method. One unit of enzyme activity was defined as the amount releasing 1 μmol of reducing sugar per minute. Adding EM temporarily makes cellulase activities increased. Cellulase activities are related to fungi and EM concentrations. As so cellulase activities are prone to influence of concentrations of contained EM.

키워드

저자

• Ha-Na OH [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]
• I-Seul MOON [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]
• Kwang-Bae LEE [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]
• Syamsul BAHRI [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]
• Young-Jun KIM [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]
• Yoon-Mo KOO [ Department of Biological Engineering, Inha University, Inha University, Incheon 402-751, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576