Earticle

초록

영어

Gene expression involves the modification and mobilization of nucleosomes, the fundamental unit of chromatin.
Nucleosomes are mobilized by chromatin remodeling complexes to alter DNA-histone contact and reveal DNA elements recognized by transcriptional factors. RSC is a nucleosome-remodeling complex essential for growth that can alter histone-DNA interaction by using the energy of ATP hydrolysis. The yeast RSC complexes contain RSC8. To confirm the existence of the complex, we tagged Rsc8 with FIAG tag and purified them in A. nidulans. Also, the rsc8 conditional mutant strain was generated to test the intracellular localization pattern and its function. This mutant has only one full-length rsc8 gene under the control of inducible promoter, alcA(p), and a truncated 5' rsc8 sequence. When mCherry-RSC8 fusion was induced on media containing glycerol as the sole carbon source, RSC8 produced a smaller colony size compared to the control R153 strain. To test whether rsc8 mutation would suppress the loss-of-function sidB mutation, we generated the sidB-rsc8 double mutant. On rich media containing glucose, the rsc8 mutation showed lethal phenotype indicating that the rsc8 mutation didn’t suppress the sidB mutation. On minimal media containing glycerol as the sole carbon source, the rsc8 mutation partially suppress the sidB mutation. Also, we checked its protein localization pattern. As a result, rsc8 localized at nucleus. In this observation, we confirmed the RSC8 mutation did not affect activity of SIDB.

키워드

저자

• Hwan Gyu KIM [ Division of Biological Sciences, Chonbuk National University, Chonju, 561-756. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576