Earticle

초록

영어

The soil bacterium Bradyrhizobium japonicum induces nodulation on the soybean root, resulting in formation of root nodules where bacteria fix atmosphereic nitrogen into ammonia. Rhizobial lipopolysaccharides (LPS) have been known to be an important factor for initiating and maintaining a symbiotic relationship with their host. In previous studies, a LPS gene region was isolated from B .japonicum strain 61A101C. In this study, we have selected one of the genes whose sequence is highly homologous to lpcC gene, encoding mannosyl transferase. We constructed lpcC knock out mutant to characterize the functions of the lpcC gene in B. japonicum. The lpcC null mutant showed distinct phenotypes from the wild type. The mutant failed to elicit nodules through in vivo plant nodulation test. To examine if LPS deficient mutant becomes sensitive to plant derived defense substance, especially to reactive oxygen species (ROS), wild type and the mutant strains were challenged with hydrogen peroxide at concentrations of 10-15μM. The mutant exhibited a significantly increased sensitivity to hydrogen peroxide. Further studies on the relationship between plant defense mechanism and nodulation are required.

키워드

저자

• Sang-Hyun HAN [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• Yong-Ha IN [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• SuYeon JEONG [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• Seong Geun KIM [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• Kwan Young KIM [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• Woo-Suk CHANG [ Department of Biology, University of Texas-Arlington, Arlington, TX, USA. ]
• Jeong-Min JEON [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]
• Jae-Seong SO [ Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576