Earticle

초록

영어

Flavonoids are polyphenolic compounds that are ubiquitous in nature and are categorized according to chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins and chalcones. Over 4,000 flavonoids have been identified, many of which occur in fruits, vegetables and beverages (tea, coffee, beer, wine and fruit drinks). The flavonoids have aroused considerable interest recently because of their potential beneficial effects on human health-they have been reported to have antiviral, anti-allergic, antiplatelet, anti-inflammatory, antitumor and antioxidant activities. Twelve microorganisms were initially screened for their abilities to catalyze biotransformation of phloretin. Streptomyces avermitilis, two main products were identified in GC/MS analysis. They were interpreted as hydroxylated products of phloretin in A-ring at different position. (mass increase 179 → 267, 192 → 280) This result confirmed hydroxylaion considering BSTFA derivatization of hydroxylated product.
Maximum conversion was 6.7 %, which was achieved for 1 hours of reaction, and the substrate (phloretin) and reaction product was completely metabolized after 3 hours of reaction. Three kinds of Cytochrome P450 inhibitor, Coumarin, Erythromycin and Quinidine was added with 0.5mM final concentration to find out the role of P450 enzymes. P450 amplifeid and deleted Streptomycs were also used to confirm the role of P450 enzyme.

키워드

저자

• Woo-Il KIM [ Department of Chemical Engineering, Dong-A University, Busan, Korea. ]
• Chang-Su LEE [ Department of Chemical Engineering, Dong-A University, Busan, Korea. ]
• Jong-hwa PARK [ Department of Chemical Engineering, Dong-A University, Busan, Korea. ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, SNU, Seoul, Korea. ]
• June-Hyung KIM [ Department of Chemical Engineering, Dong-A University, Busan, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576