Earticle

초록

영어

2,3-Butanediol(2,3-BDO) is a key starting material for manufacture of bulk chemicals such as methyl ethyl ketone and 1,3-butadien. It also has potential applications in the manufacture of printing ink, perfumes and explosive. Microbial production of 2,3-BDO by using Klebsiella pneumoniae was first investigated in 1906. Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose fermenting, facultative anaerobic, rod shaped bacterium found in the normal flora of the mouth, skin, and intestines. In this study, in order to enhanced 2,3-BDO production, budC gene coding for butanediol dehydrogenase was over-expressed and ldhA gene coding for lactate dehydrogenase disrupted by inserting a tetracycline resistance marker in Klebsiella pneumoniae KTCT2242. The fermentation results of both the parent and mutant strain suggested that over-expression of budC and inactivation of ldhA resulted in an improved 2,3-BDO production.

키워드

저자

• Bo-Rim KIM [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]
• Woojoo CHOI [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]
• Doyeon KWON [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]
• Jinwon LEE [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576