Earticle

초록

영어

Span 80 was selected among the tested detergents for retinal production by metabolically engineered E. coli because the addition of Span 80 increased significantly retinal production. In the flask culture, the optimum concentration of span 80 was determined as 5 g l–1. In the batch culture with 5 g l–1 Span 80, the cell mass, retinal concentration, retinal content, and retinal productivity were 20 g l–1, 61 mg l–1, 3.5 mg g-cells–1, and 3 mg l–1 h–1 after 22 h, respectively. In the fed-batch culture of glycerol with 5 g l–1 span 80, the cell mass, retinal concentration, retinal content, and retinal productivity were 38 g l–1, 335 mg l–1, 8.8 mg g-cells–1, and 16 mg l–1 h–1 after 21 h, respectively.
They were approximately 1.1, 3.0-, 2.6- and 3.2-fold higher than those in the fed-batch culture of glycerol without 5 g l–1 Span 80, respectively.
This is the first report of retinal production by using the metabolically engineered E. coli. Furthermore, retinal productivity was successfully enhanced by the supplement of Span 80.

키워드

저자

• Jae-Hee Lee [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Yeong-Su Kim [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Chang-Su Park [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Seon-Won Kim [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea. ]
• Deok-Kun Oh [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576