Earticle

초록

영어

Streptomycetes, a major family of actinomycetes, has been intensively investigated, but a potentially-valuable non-streptomycetes rareactinomycetes (NSRA) family is largely uncharacterized. A PCR-based streptomycetes-specific screening was performed to isolate NSRA strains from actinomycetes strain collection which is 180 independentlyisolated antifungal actinomycetes strains (kindly provided by KRIBB) [1, 2]. After screened out most of streptomyces species from the strain collection, several remaining NSRA strains were then confirmed and classified based on 16S rRNA sequence information. To identify the stains whose genes might associate with production of valuable secondary metabolite, PCR-based genome screening was performed using established genetic information such as typeⅠ PKS and NRPS.
One strain, putative owning typeⅠ PKS and NRPS genes, shows both antifungal activity and antibacterial activity among the selected strains.
These results suggest that a PCR screening strategy for not only NSRA strains but also NSRA genetic manipulation might lead to the isolation of potentially-valuable biosynthetic genes and compounds present in various rare-actinomycetes.

키워드

저자

• Ho-Geun JEON [ Department of Biological Engineering, Inha University, Incheon. ]
• Mi-Jin LEE [ Department of Biological Engineering, Inha University, Incheon. ]
• Eung-Soo KIM [ Department of Biological Engineering, Inha University, Incheon. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576