Okkyoung CHOI, Byung Chun KIM, Ji Hae AHN, Youngsoon UM, Byoung-In SANG, Yong Hwan KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129254
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원문정보
초록
영어
Biomimetic synthesis raises a concern because it can take place under mild conditions with more facilitation (1). Biosilicification is one of biomimetic syntheses and we recently efficiently immobilized GFP (green fluorescence protein) using biosilicification by silaffin-fused proteins (GFP-Rx) (2). Glucose oxidase (GOx, EC 1.1.3.4) is one of broadly used enzymes for biosensors with high enzyme activity to detect glucose concentration such as to track blood sugar levels. We constructed R5-GOx-fused protein and it was expressed on Pichia pastoris. Secreted GOx from Pichia was immobilized by R5 (fused with GOx) in pH 5 citric buffer using 0.1 M tetramethoxysilane. To apply silaffin-GOx-silica nanoparticles for a biosensor, biosilicification occurred on a grassy carbon electrode. Electric cell consisted of Ag/AgCl reference electrode, platinum counter electrode (cathode), and working electrode (anode) modified with poly(neutral red)(PNR)/GOx/nafion. Electrochemically formed PNR was used as an electron carrier from GOx to an electrode. Scanning electron microscopy image supported a formation of PNR/GOx complex. A current was increased to 2 mA·cm-1 by 1 mM glucose at -0.4 V applied potential. A biosensor of GC/PNR/GOx/nafion showed a substrate selectivity to detect glucose concentration in mixed glucose samples with other carbohydrates.
키워드
BiosensorGlucose oxidaseBiosilicificationsilaffin-fused protein
저자
Okkyoung CHOI [ Clean Energy Center, Korea Institute of Science and Technology, Seoul, 136-791. ]
Byung Chun KIM [ Clean Energy Center, Korea Institute of Science and Technology, Seoul, 136-791. ]
Ji Hae AHN [ Clean Energy Center, Korea Institute of Science and Technology, Seoul, 136-791. ]
Youngsoon UM [ Clean Energy Center, Korea Institute of Science and Technology, Seoul, 136-791. ]
Byoung-In SANG [ Clean Energy Center, Korea Institute of Science and Technology, Seoul, 136-791. ]
Yong Hwan KIM [ Dept. of Chemical Engineering, Kwangwoon University, Seoul, 139-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동