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포스터 3분 Speech : 좌장 : 최신식(명지대), 양영헌(건국대)

Medium Optimization for Exopolysaccharides Production from Aureobasidium pullulans using Statistical Experimental Methods

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2010 추계학술대회 및 국제심포지움 (2010.10)바로가기
  • 페이지
    pp.154-154
  • 저자
    Sang-Young YOON, Eun-Soo HONG, So-Hyun KIM, Pyung-Cheon LEE, Min-Soo KIM, Yeon-Woo RYU
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A129251

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원문정보

초록

영어
Exopolysaccharides(EPS) from Aureobasidium pullulans are used for health foods and cosmetics. In order to better exploit EPS production from A. pullulans, medium composition was optimized using stastical methods. And, effect of each nutrient of basal medium was investigated by preliminary studies (one-factor-at-a-time). Maximum growth and EPS production were observed at various initial pH 3-5, while growth and EPS production were also very low at pH 2 and pH 7. Among various nitrogen sources (Ashbya gossypii extract, beef extract, corn steep liquor, corn steep, powder, malt extract, soytone peptone and yeast extract) tested, the highest mycelial growth and EPS production were achieved in the medium containing Ashbya gossypii extract. Also, among various carbon sources, EPS production and the growth of A. pullulans was the best on sucrose. The Plackett–-Burman design was used to evaluate the effects of 7 variables selected from preliminary studies, and three statistically significant variables namely, sucrose, NaNO3, and Ashyba gossiipii extract, were selected.
Then, a three-level Box-Behnken design was employed to optimize the medium composition for EPS production in shake-flask. Using this methodology, the quadratic regression model of producing EPS was built and the optimal combinations of media constituents and culture conditions for maximum EPS production (about 30g/L) were determined.

저자

  • Sang-Young YOON [ Dept. of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Yeongtong-gu, Suwon, 443-749, Korea. ]
  • Eun-Soo HONG [ Dept. of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Yeongtong-gu, Suwon, 443-749, Korea. ]
  • So-Hyun KIM [ Dept. of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Yeongtong-gu, Suwon, 443-749, Korea. ]
  • Pyung-Cheon LEE [ Dept. of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Yeongtong-gu, Suwon, 443-749, Korea. ]
  • Min-Soo KIM [ Jeonbuk Branch Institute Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Korea. ]
  • Yeon-Woo RYU [ Dept. of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Yeongtong-gu, Suwon, 443-749, Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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