Thai-Hoang LE, Sung-Kyu LEE, Yang-Hoon KIM, Jiho MIN
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129242
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
This study will attempt to clarify the impact of two pharmaceuticals (i.e. verapamil, tramadol) and two pesticides (i.e., glyphosate, methidathion) on non-target organism in aquatic environment. We investigated the toxic effects of these chemicals on physiological responses such as viability, growth, and reproduction as well as molecular responses such as gene expression in water flea, Daphnia magna. Through RT-PCR, the expression of five genes selected as representative biomarkers such as Arnt, Vtg, CYP4, CYP314 and Dhb was analyzed in D. magna exposedto hazardous materials. Our results showed that the expression of these genes in D. magna were significantly down-regulated in response to pesticides. Among five examined genes, Cyp4 and Cyp314 genes, expressed differently in exposure of glyphosate and methidathion, respectively. This revealed that these two genes could be considered as potential biomarkers. Unlike pesticides, pharmaceuticals did not significantly affect on gene expression in D. magna in short-time exposure, but inhibited the expression of Vtg gene, a biomarker of animal reproduction, after 21d exposure. In addition, total number of D. magna neonates decreased strongly in the chronic toxicity test by both kinds of pharmaceuticals. Therefore, this study suggests that these pharmaceuticals and pesticides have distinguishable toxic modes between them. While the pesticides cause molecular damages such as significantly reduced of five genes, the pharmaceuticals lead to a chronic toxicity; not the effect of gene expression caused by acute exposure.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동