Earticle

초록

영어

In this study, we developed a novel protein-free medium for CHO DG44 host cells in suspension culture. Chinese hamster ovary (CHO) DG44 host cell line is mainly used in the production of monoclonal antibody (mAB) for therapeutic use on the market. Serum has been used in culture of recombinant CHO cells for mAB production, but since serum possesses potential contaminants, e.g. viruses, mycoplasma or prions, commercial protein-free media with serum eliminated are mostly used in mAB production. The composition of commercial media is unknown and the suspension culture of rCHO DG44 cells with commercial media requires long term adaptation periods. Development of an in-house protein-free medium with stable cell growth at adaptation-free or short term adaptation for CHO DG44 host cells will enable to overcome these limitations. We have established the formulation of protein-free components such as amino acids, iron supplement, hydrolysate, energy source, inorganic salts, etc. The maximum cell density of the medium was 7.5*106 cells/ml in batch culture with 52% higher cell growth than the commercial media.

키워드

저자

• Bong-Gyun KIM [ Division of Applied Chemical Engineering and Bio Engineering, Hanyang University, Seoul, 133-791. ]
• Hong-Woo PARK [ Division of Applied Chemical Engineering and Bio Engineering, Hanyang University, Seoul, 133-791. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576