The changes in bacterial communities associated with volatile fatty acids (VFAs) profiles were investigated in anaerobic batch reactors treating swine wastewater. Denaturing gradient gel electrophoresis (DGGE) and real-time QPCR were used to analyze the bacterial community structure and the methanogenic population, respectively. The three subspecies of Lactobacillus delbrueckii (bulgaricus, lactis, and indicus)along with the Clostridium-like organismswere identified.The L. delbrueckii-related organisms were assumed to be responsible for the fermentation of the organic compounds throughout the overall reaction period. The presence of C. ultunense and C. kluyveri, together with hydrogenotrophic methanogens, was essential for methane production from propionic acid in the later stage of the experiment. The order Methanomicrobiales, hydrogenotrophic methangoens, increased rapidly as the process proceeded, resulting in its dominance above 64% after day 28, among the methanogenic groups monitored. Our results showed that the variations in the VFA concentrations and methane production were complicated and dynamic. It was also demonstrated that analyzing acidogenic community along with methanogenic population was essential for better insights into understanding anaerobic digestion of swine wastewater.
키워드
volatile fatty acidsacidogensmethanogensDenaturing gradient gel electrophoresisreal-time QPCR
저자
Kwanghyun HWANG [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
Sulhee YOO [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
Huijuan LI [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
Woong KIM [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
Minkyung SONG [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
Seokhwan HWANG [ School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Namgu, Pohang, Gyungbuk 790-784, Republic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동