The optimal fed-batch process for high cell density culture of E.coli K-12/pHCE-InaN-GAPDH-Ghost27 as a ghost vaccine was developed. The nutrient feeding strategy with Riesenberg's defined medium during fed-batch process was evaluated. The fermentation was conducted in four phases as follow: initial phase (phase 1), fed-batch phase (phase 2), temperature increase phase (phase 3) and high temperature holding phase (phase 4). The maximum ghost bacterial vaccine (GBV) was obtained about 38 g dcw/L (OD600=100). The efficacy of GBV was evaluated by the challenge test with the infection of live S. iniae to vaccinated olive flounders. E. coli K-12 host strain group, E. coli K-12/pHCE vector control group and fomalin-killed cell (FKC) treated vaccine group showed 100%, 100% and 65% of cumulative mortalities, respectively. The GBV treated groups showed 50% of cumulative mortalities with increased survival ratio. Hence, the immunoprotective efficacy of GBV against S. iniae was better than that of FKC vaccine. Therefore, the GBV could be used as an effective and vaccine in aquaculture for the prevention of streptococcal disease.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동