The application of nanoparticles (NPs) in various fields has been growing rapidly due to its exceptional physicochemical properties. The potential biological or environmental toxicity of NPs, however, has been frequently reported. In this study, the toxicity of nC60 and nC60-OH NPs in Caenorhabditis elegans was assessed through the measurement of lifespan and brood size. The water-stable colloids form of nC60 NPs are prepared through the long-term exposure of fullerene, C60 in toluene. C. elegans is a free-living soil nematode feeding on bacteria such as Escherichia coli. We confirmed that the oral-administrated NPs were accumulated in the animals for several days after feeding the mixture of bacterial food and nC60 or nC60-OH NPs to the L4 larvas of C. elegans. The nC60-OH NPs reduced the viability of animals while no decrease in survival was found in the animals fed with nC60. We have also found that both nC60 and nC60-OH reduced the reproduction of C. elegans. In order to illuminate the genetic mechanism of toxicity induced by those NPs, the assessment of viability and fertility was carried out using the strains mutated in oxidative stress or programmed cell death regulators.
키워드
c. elegansnanoparticletoxicity
저자
Yun Jeong CHA [ Dept. of Food and Nutrition, Myongji University, Yongin, Kyonggido, 449-728. ]
Shin Sik CHOI [ Dept. of Food and Nutrition, Myongji University, Yongin, Kyonggido, 449-728. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동