Earticle

초록

영어

Recombinant bovine trypsin was produced in transgenic rice cell suspension culture using the rice α-amylase system. The effects of alternative carbon sources on production of recombinant trypsin were investigated in transgenic rice cell suspension cultures. While this expression system is inducible by sugar depletion, we have found that the productivity of recombinant trypsin increased 5.4 to 6.3 fold, compared with the control medium without carbon source and NaCl. The accumulated recombinant trypsin in the medium containing fumaric acid and NaCl reached 79.5 – 95.3 mg/L on day 7 after sugar starvation. By adding the alternative carbon source and NaCl in the induction medium, the percentage of the active recombinant trypsin in the total secreted proteins increased from 20 to 40.3%. Supplementation with these alternative carbon sources has potential for high production of recombinant enzymes in rice cell suspension culture. Acknowledgments This work was supported by a grant from the Next Generation New Technology Development program of the Ministry of Commerce, Industry and Energy, and by the National Research Foundation of Korea Grant funded by the Korean Government (2009-0070005)

키워드

저자

• Hwa-Young YU [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]
• Nan-Sun KIM [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]
• Tae-Ho KWON [ Jeonju Bio-materials Institute, Jeonbuk 561-360. ]
• Moon-Sik YANG [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576