Earticle

초록

영어

Photosynthetic microorganisms produce relatively large amounts of physiological activation materials and stimulate the physiological activity of other organisms. In this study, two mammalian cell were cultured under the different culture medium, Dulbecco’'s Modified Eagle medium (DMEM) including fetal bovine serum (FBS) and DMEM including physiological activation materials produced from photosynthetic microorganism. Cell growth was estimated by cell counting in a hemocytometer. The level of cellular stress was measured using LysoTracker dyes, known as a specific fluorescent dye to stain only lysosome. In comparison with FBS, we did not show significant differences for growth of mammalian cell under the feed condition with physiological activation materials. The growth of the cells can be similarly controlled by varying the photosynthetic microorganism concentration. In addition, we found that any cellular stresses were not induced by adding the physiological activation materials in mammalian cells. Our results suggest that new materials produced from photosynthetic microorganism can enhance the physiological activity of mammalian cells as a nontoxic bioavailable materials.

키워드

저자

• Hyun Jeong LEE [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756. ]
• Ju-Yong PARK [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756. ]
• Yang-Hoon KIM [ School of Life Science, Chungbuk National University, Cheongju 361-763. ]
• Jiho MIN [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576