This research illustrates the development of microbial production of fatty acid, which can be transformed into the bio-diesel for alternative energy. We developed a new green technology that can produce a fatty acid with high productivity from both strains, Bacillus subtilis (gram-positive) and Escherichia coli (gram-negative). To produce a fatty acid in bacteria, it is essential to generate a three-carbon precursor, malonyl-CoA, which is derived from acetyl-CoA by the action of acyl-CoA carboxylase (accA, accB, accC). The malonyl-CoA is transferred to the acyl carrier protein by malonyl-CoA:ACP acyltransferase (FabD). This process is the most essential step in the biosynthesis of long–-chain fatty acids. Therefore, overexpression of accA, accB and accC genes, encoding acetyl-CoA carboxylase subunits enzymes, is expected to increase the productivity of fatty acids. The single, double and triple organized artificial operons of accA, accB, accC and fabD were designed and constructed in a vector. By introducing four distinct genes obtained from Bacillus subtilis into E. coli, we have engineered E. coli strain for high productivity of fatty acids.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동