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Homogeneous Colorimetric Assay of Oxytetracycline using DNA Aptamer Adsorbed Unmodified Gold Nanoparticles

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2010 추계학술대회 및 국제심포지움 (2010.10)바로가기
  • 페이지
    pp.129-129
  • 저자
    Yeon Seok KIM, Joong Hyun KIM, In Ae KIM, Su Jin LEE, Jongsoo JURNG, Man Bock GU
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A129200

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원문정보

초록

영어
We have successfully developed a homogeneous colorimetric assay using DNA aptamer and unmodified gold nanoparticles (AuNPs) for sensitive and specific detection of oxytetracycline, one of most commonly used antibacterial agents.1 A highly specific DNA aptamer that bind to oxytetracycline with high affinity was employed to discriminate other structurally similar antibacterial agents in tetracycline's group, such as tetracycline and doxycycline. Oxytetracycline binding DNA aptamers protected the aggregation of AuNPs at high salt concentration by the adsorption on the surface of AuNPs in the absence of oxytetracycline.2 The aggregation of AuNPs was induced by desorption of oxytetracycline binding aptamers on the AuNPs as a result of the interaction between aptamers and oxytetracycline, leading to the color change from red to purple.
The color change of mixture was observed as low as 1 uM oxytetracycline with naked eyes, but other antibacterial agents didn't induced the color change. The detection limit of oxytetracyline was enhanced up to 25 nM by UV spectrometer analysis, which was 20-fold low than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the salt and AuNPs, and the molar ratio of aptamers to AuNPs. This colorimetric assay is advantageous over the other conventional methods in terms of its simple signal generation and detection with naked eyes, which can be realized in on-site detection of antibacterial oxytetracycline.

저자

  • Yeon Seok KIM [ Environmental Division, Korea Institutue of Science and Technology, Seoul, Korea. ]
  • Joong Hyun KIM [ BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. ]
  • In Ae KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
  • Su Jin LEE [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
  • Jongsoo JURNG [ Environmental Division, Korea Institutue of Science and Technology, Seoul, Korea. ]
  • Man Bock GU [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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