Earticle

초록

영어

Marine mussels attach to substrates using adhesive proteins. Mussel adhesive proteins (MAPs) have received increased attention as potential biomedical and environmentally friendly adhesives. However, practical applications of MAPs have been severely limited by uneconomical extraction and unsuccessful genetic production. Developing new adhesives requires access to large quantities of material and demonstrations of bulk mechanical properties. Previously, we designed fp‐151, a fusion protein comprised of six MAP type 1 (fp‐1) decapeptide repeats at each MAP type 5 (fp‐5) terminus and successfully expressed it in Escherichia coli. This recombinant hybrid protein exhibited high level expression, a simple purification, and high biocompatibility as well as strong adhesive ability on a micro scale. In the present work, we performed investigations on the bulk adhesive properties of purified fusion fp‐151 in air. The unmodified recombinant fp‐151, as expressed, contains tyrosine residues and showed significant shear‐adhesive forces. Adhesion strength was increased after enzymatic oxidation of tyrosine residues to L‐3,4‐dihydroxyphenylalanine (DOPA) groups. Addition of cross‐linkers generally enhanced adhesion, although too much addition decreased adhesion. Taken together, we present the first bulk‐scale adhesive force measurements for an expressed recombinant hybrid mussel adhesive protein. It has also been suggested that complex coacervation (liquid‐liquid phase separation via concentration) might be involved in the highly condensed and non‐water dispersed adhesion process of MAPs. However, as purified natural MAPs are difficult to obtain, it has not been possible to experimentally validate the coacervation model. In the present work, we demonstrate complex coacervation in a system including recombinant MAPs and hyaluronic acid (HA). We observed successful complex coacervation using cationic MAPs and an anionic HA partner.
Importantly, we found that highly condensed complex coacervates significantly increased the bulk adhesive strength of MAPs in both dry and wet environments. Collectively, our results indicate that a complex coacervation system based on MAPs shows superior adhesive properties, combined with additional valuable features including liquid/liquid phase separation and appropriate viscoelasticity.

저자

• Hyung Joon CHA [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Seonghye LIM [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576