Monosaccharides have recently attracted much attention due to their many uses, such as low-calorie sweeteners, bulking agents, immunosuppressants in allogenic orthotopic liver transplantation, and potential inhibitors of various glycosidases, ischemia/reperfusion injury of the rat liver, and segmented neutrophil production without other detrimental clinical effects. Monosaccharides can be made by microbial and enzymatic reactions with ketose epimerase, aldose isomerase, aldose-phosphate isomerase, aldose reductase, and oxidoreductase. Among sugar converting enzymes, sugar isomerases including L-arabinose isomerase, L-rhamnose isomerase, L-fucose (D-arabinose) isomerase, and D-lyxose isomerase; and sugar-phosphate isomerases including D-ribose-5-phophate isomerase, D-mannose-6-phophate isomerase, D-glucose-6-phophate isomerase, and D-galactose-6-phophate isomerase are of interest for the production of monosaccharides because they have various types of substrate specificities. In this study, the biochemical properties and substrate specificities of sugar isomerases and sugar-phosphate isomerases are investigated and the production of monosaccharides is attempted using these enzymes. Increases in the production of monosaccharides are realized by genetic improvements via directed evolution and by structural modification of the determinant residues on or near the active site, based on homology models or the determined structure of the enzymes.
저자
Deok-Kun OH [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동