Xylose reductase (XR) and Xylitol dehydrogenase (XDH) are important enzymes for transforming hemicellulosic material, xylose, to fermentative sugar. Although several xylose-utilizing recombinant microbes of Saccharomyces cerevisiae (S. cerevisiae) have been constructed by genetically expressing XDH and XR, the results were not successful. In particular, the utilizing rate of xylose for ethanol production in recombinant S. cerevisiae was not sufficient, so that enzymatic functionalities of XR and XDH should be improved by protein engineering approach. In this study, we designed XR and XDH from Pichia stipitis (PsXDH) in terms of their cofactor preferences and thermostability. In particular, we developed appropriate design strategies via bioinformatical comparison with other polyol dehydrogenase family members. More functional PsXDH and PsXR varients were obtained by site-directed mutagenesis. Recombinant S. cerevisiae expressing the designed PsXR or PsXDH showed high perfoemance in xylose-toethnaol production.
저자
Seung Pil Pack [ Department of Biotechnology and Bioinformatics, Korea University, Korea. ]
Keisuke Makino [ Institute of Advanced Energy, Kyoto University, Kyoto, 611-0011, Japan. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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