Earticle

초록

영어

The typical chemical structure of lipids is relatively simple, but lipid diversity is very high owing to complexity arising from combinations of hydrophobic acyl chain molecular species. Generally, lipids are considered to be hydrophobic compounds. However, their hydrophilicity occasionally increases when combined with highly hydrophilic molecules such as phosphoric acid and sugar. Thus, lipids possess a wide range of polarity. Sophisticated techniques are required for separation and component analysis to identify each lipid in a lipid mixture. A supercritical fluid is a substance whose temperature and pressure are above the critical values, and which possesses features such as low viscosity and high diffusivity. Therefore, it exhibits properties suitable for its application as a mobile phase for chromatography. SFC is a suitable technique for the separation of hydrophobic compounds, and is superior to liquid chromatography/mass spectrometry (LC/MS) because of its high throughput and high resolution. Therefore, we attempted to apply SFC/MS to lipid profiling in order to establish an analysis system applicable to lipid metabolomics. Separation conditions for SFC (column, modifier, back pressure, etc.) and detection conditions in the mass spectrometer (ionization method, parameters, etc.) were investigated in order to develop a simultaneous analytical method for lipid mixtures comprising phospholipids, glycolipids, neutral lipids, and sphingolipids. When a cyanopropyl silica gel packed column was used for the separation, all the lipids were successfully detected, and the analysis was completed in less than 15 min. The use of an octadecylsilyl column resulted in separation based on the differences in the unsaturation of the fatty acid side chains and isomer distribution. Additionally, SFC/MS was applied for profiling lipids in soybeans. For this purpose, lipids extracted from 12 soybean cultivars were used. Different soybean cultivars used in the manufacture of various processed foods, such as natto, tofu, edamame, and nimame, could be distinguished from each other. In principal component analysis (PCA), triacylglycerol (TAG) was found to be the main variable for the discrimination of soybean cultivars. For a more effective discrimination of soybean cultivars, a detailed TAG profiling method was developed by using SFC/MS with 3 Chromolith Performance RP‐18e columns connected in series. TAG molecules were separated effectively within 8 min without purification. Additionally, each TAG was successfully identified by using programmed cone voltage fragmentation, even without MS/MS analysis and any standard sample. This study shows that SFC/MS is a powerful tool for studies on lipid metabolomics because it is useful not only as a fingerprinting method for screening diverse lipids, but also for the detailed profiling of individual components.

저자

• Takeshi BAMBA [ Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576