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IBE-KSBB Joint Symposium : Chair: Mark R. RILEY(The University of Arizona, USA), Hei Chan LEE(SunMoon University, Korea)
Visual Detection of UV-induced Mutagenic DNA Photodimers using Gold Nanoparticles
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2010 추계학술대회 및 국제심포지움 (2010.10)바로가기
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- 페이지
- pp.82-82
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A129145
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The two major forms of the pyrimidine dimers are known to be the cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidone (6-4) photoproduct. CPD the 6‐4 photoproduct of thyminehave been reported to cause structural changes of DNA, such as bending or kicking by 30℃ and 40℃, respectively. The conventional methods using circular dichroism spectroscopy and atomic force microscopy for monitoring the structural deformation of DNA are time consuming and instrument oriented. In recent, we observed red to purple color change of AuNPs only after mixing with UV‐irradiated DNA in a high salted buffer. The color changes of the mixture was dependent on UV irradiated time and base compositions of the UV‐irradiated DNA. The stability of gold nanoparticles in a high ionic strength solution is maintained by straight ssDNA adsorbed physically on the AuNPs. Single‐stranded and striated DNAs are known to adsorb onto AuNP by uncoiling themselves to facilitate electrostatic interactions between the bases and AuNPs. The UV irradiation to DNA accumulated a conformational deformation of the DNA structure by multiple‐dimer formation. However, the covalent bonds formed in the pyrimidine dimers could impose more stiffness on the UV irradiated DNA. As a result, the UV‐irradiated DNA became more rigid and thus could not effectively uncoil the compacted structure for the electrostatic interaction with AuNP and resulted in AuNP aggregation under the high salt condition. This hypothesis was supported and confirmed with no observation of any mass of fragmented DNA or radical oxygen species under the UV irradiation. It has been also known that the pyrimidine dimers are formed as result of triplettriplet energy transfer by exogenous drugs. Because our method does not require any chemical or biochemical treatments or special instruments for purifying and qualifying the DNA photolesions, it should provide a feasible tool for the studies of the UV‐induced mutagenic or carcinogenic DNA dimers and accelerate screening of a large number of drug candidates.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
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