Joo‐Myung Ahn, Joong Hyun Kim, Ji Hoon Kim, Man Bock Gu
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129104
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원문정보
초록
영어
We have successfully developed optically coded functional microbeads by coencapsulating both bioluminescent reporter bacterial cells and fluorescent microspheres within a common alginate microbead [1]. This smart functional microbead has a specific stress‐specific bacterial strain and, as an its identification optical code, one of five optical codes generated from fluorescence microspheres such as yellow, green, red, yellow + green, or no fluoresce. These smart microbeads could be randomly scattered on any multi‐well chip plate with the result that, since the cell types are identified on the basis of fluorescent color, the microbead arrays were fabricated without pre‐designation of an individual well. As an example of this method, five different stress specific bioluminescent bacterial strains, each with a different optical code, were successfully implemented to make five different types of optically coded functional microbeads, with a speed of about 30 microbeads/min. This final randomly scattered functional microbeads array biochip, with a fast fabrication of each chip at every 2 min, successfully demonstrated its ability in toxicity screening and monitoring for samples with a few examples for five different stress chemicals. This simple and fast, but not tedious and complicated procedure should be widely and practically used in making the cell array chips for the monitoring of environmental toxicity, new‐borne chemicals, pharmaceutical drugs and the cosmic rays in space station or spaceships in future.
저자
Joo‐Myung Ahn [ College of Life Sciences and Biotechnology, Korea University, equally contributed ]
Joong Hyun Kim [ BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea, equally contributed ]
Ji Hoon Kim [ College of Life Sciences and Biotechnology, Korea University Republic of Korea ]
Man Bock Gu [ College of Life Sciences and Biotechnology, Korea University, Republic of Korea ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동