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말 정액 동결시 Glycerol 농도와 동결 속도가 생존율에 미치는 영향
Factors Affecting the Survival Rates of Frozen-Thawed Spermatozoa in Equine by Glycerol Concetration and Freezing Speed

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 34 No 3 (2010.09)바로가기
  • 페이지
    pp.271-274
  • 저자
    최선호, 김성재, 조상래, 최창용, 손준규, 유용희, 조영재, 최귀철, 문윤영
  • 언어
    한국어(KOR)
  • URL
    https://www.earticle.net/Article/A128425

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원문정보

초록

영어
This study was conducted to investigate the survival rate of frozen-thawed spermatozoa in equine by glycerol concentration and freezing speed. Two stallions (1 Thoroughbred-13 year old and 1 Arab-7 year old) bred in Korea Racing authority was examined for 1 times in a couple of weeks. Semen was collected by condom method standing heated mare and were centrifuged 650 g for 15 min. and isolated the seminal plasma. Thick fraction of semen was diluted EDTA-Lactose-egg yolk diluents to 1:1 and contained in 0.5 ml straw as 6~14×107cells/ml. Final concentrations of glycerol were 3, 5 and 7% in cryopreseved diluents and added 4 times for 2 hours equilibration. For the freezing, equilibrated straws were located 3 or 5 cm above LN2 gas for 5 or 10 min. Survival rates of pre-frozen sperm were 65.0±13.2%, 68.3±10.4%, 66.7±11.5% and post-frozen were 53.3±23.1%, 45.0±15.0%, 50.0±18.0% in 3, 5, 7% glycerol concentration, respectively. There was no difference between glycerol concentrations. Survival rates of frozen-thawed sperm on freezing speed were 36.7±10.4%, 40.0±7.1%, 30.0±13.2% at 3 cm-5 min and 33.3±11.5%, 31.7± 2.9%, 21.7±10.4% at 3 cm-10 min in 3, 5, 7% glycerol concentration, respectively. Survival rates of frozen-thawed sperm on freezing speed were 43.3±15.3%, 32.0±17.9%, 22.3±15.7% at 5cm-5 min and were 47.5±15.0%, 43.3±12.6%, 48.3±15.3% at 5cm-10 min in 3, 5, 7% glycerol concentration, respectively. There were significantly different between groups (p<0.05). These results suggest that glycerol concentration did not affect cryopreservation of stallion semen within 3~7% but freezing speed affects. In our experiment, the best cryopreservation condition was at 5 cm above LN2 gas for 10 min for pre-freezing and 7% of glycerol concentration. These results lead to commercial AI with frozen-thawed stallion semen.

목차

ABSTRACT
 서론
 재료 및 방법
  공시동물
  정액 체취
  정액검사
  희석액의 제조 및 정액의 동결-융해
  통계처리
 결과
  Glyeerol 농도에 따른 동결-융해 정액의 생존율
  동결 속도에 따른 동결-융해 정액의 생존율
 고찰
 인용문헌

키워드

Survival rate Frozen-thawed Stallion Sperm

저자

  • 최선호 [ Cun-Ho Choi | 농촌진흥청 국립축산과학원 ] Corresponding author
  • 김성재 [ Sung-Jae Kim | 농촌진흥청 국립축산과학원 ]
  • 조상래 [ Sang-Rae Cho | 농촌진흥청 국립축산과학원 ]
  • 최창용 [ Chang-Yong Choe | 농촌진흥청 국립축산과학원 ]
  • 손준규 [ Jun-Kyu Son | 농촌진흥청 국립축산과학원 ]
  • 유용희 [ Yong-Hee Yoo | 농촌진흥청 국립축산과학원 ]
  • 조영재 [ Young-Jae Cho | 한국마사회 ]
  • 최귀철 [ Gui-Cheol Choi | 한국마사회 ]
  • 문윤영 [ Yun-Young Moon | 한국마사회 ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 34 No 3

    피인용수 : 0(자료제공 : 네이버학술정보)

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