Earticle

초록

영어

Luteal cells produce progesterone that supports pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. In the present study, the corpus luteum (CL) in early pregnancy established from luteal phase and pregnant phase was analyzed. The first study determined progesterone changes in the bovine CL at day 19 (early maternal recognition period) and day 90 in mid-pregnancy and compared them to the CL from day 12 of the estrous cycle. CL alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing CL from luteal phase to those from pregnant phase counterparts, significant changes in expression level were found in 23 proteins. Of these proteins 17 were not expressed in pregnant phase CL but expressed in luteal phase counterpart, whereas, the expression of the other 6 proteins was limited only in pregnant phase CL. Among these proteins, vimentin is considered to be involved in regulation of post-implantation development. In particular, vimentin may be used as marker for CL development during pregnancy because the expression level changed considerably in pregnant phase CL tissue compared with its luteal phase counterpart. Data from 2-DE suggest that protein expression was disorientated in mid pregnancy from luteal phase, but these changes was regulated with progression of pregnancy. These findings demonstrate CL development during mid-pregnancy from luteal phase and suggest that alternations of specific CL protein expression may be involved in maintenance of pregnancy.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Experimental Design
  Preparation of Ovaries
  TReatment of Ovaries
  Measurement of Progesterone
  2-DE Polyacrylamide Gel Electrophoresis
  Statistical Analysis
 RESULTS
  Progesterone Level
  Proteomic Analysis of Cyclic and Pregnant Corpus Luteum Tissues
  Proteins Differentially Expressed in Pregnant Luteal Tissue
 DISCUSSION
 REFERENCES

키워드

저자

• Hak-Jae Chung [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ] Corresponding author
• Dong-Min You [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Hyo-Ju Kim [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Hye-Young Choi [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Myeong-Suk Lee [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Jin-Bum Kim [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Suck-Dong Lee [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Jung-Yong Park [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]
• Myeung-Sik Lee [ Molecular Animal Reproduction Lab, Hanwoo Experiment Station, National Institute of Animal Science, RDA ]

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636