Earticle

초록

영어

Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Regents
  Animals
  Preantral Follicle Dissection and Collection
  Preantral Follicle Culture In Vitro
  Experimental Design
  Statistical Analysis
 RESULTS
  Effects of FCS on Porcine Preantral Follicular Survival, Growth and Antumn Formation
  Effects of Different Culture Media (MI99 and NC-SU23) on Survival, Growth and Antrum Formation of Porcine Preantral Follicles
 DISCUSSION
 REFERENCES

키워드

저자

• Yun-Fei Diao [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Hong-Rye Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Rong-Xun Han [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Myung-Yoon Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Chang-Sik Park [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Dong-Il Jin [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636