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Efficient Labeling of Porcine Hematopoietic Cells by Fluorescence-Conjugated Nanoparticles

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 34 No 3 (2010.09)바로가기
  • 페이지
    pp.175-180
  • 저자
    Hyun-Joo Lee, Eun-Ji Park, Yong-Soo Lee, Sung-Won Park, Jae-Hwan Kim, Dong-Ku Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A128409

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초록

영어
Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Nanoparticles
  Porcine Hematopoietic Cells
  Flow Cytometry Analysis
  Cell Viability and Proliferation Assay
  Cellular Distribution of SR Nanoparticles
  Statistics Analysis
 RESULTS
  Determination of SR Nanoparticles Uptake by Porcine Hermatopoietic Cells
  Cytotoxicity and Effects of Nanoparticles
  Intracellular Uptake of Nanoparticles
 DISCUSSION
 REFERENCES

키워드

Silica-coated nanoparticles Porcine hematopoietic cells RITC Biocompatibility Confocal microscopy

저자

  • Hyun-Joo Lee [ Dept. of Biomedical Science, College of Life Science, CHA University ]
  • Eun-Ji Park [ Dept. of Biomedical Science, College of Life Science, CHA University ]
  • Yong-Soo Lee [ Dept. of Biomedical Science, College of Life Science, CHA University ]
  • Sung-Won Park [ Dept. of Biomedical Science, College of Life Science, CHA University ]
  • Jae-Hwan Kim [ Dept. of Biomedical Science, College of Life Science, CHA University ]
  • Dong-Ku Kim [ Dept. of Biomedical Science, College of Life Science, CHA University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 34 No 3

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