Hyun-Joo Lee, Eun-Ji Park, Yong-Soo Lee, Sung-Won Park, Jae-Hwan Kim, Dong-Ku Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A128409
※ 기관로그인 시 무료 이용이 가능합니다.
4,000원
원문정보
초록
영어
Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Nanoparticles Porcine Hematopoietic Cells Flow Cytometry Analysis Cell Viability and Proliferation Assay Cellular Distribution of SR Nanoparticles Statistics Analysis RESULTS Determination of SR Nanoparticles Uptake by Porcine Hermatopoietic Cells Cytotoxicity and Effects of Nanoparticles Intracellular Uptake of Nanoparticles DISCUSSION REFERENCES