Earticle

초록

영어

This study was to investigate the effect of flavonoid treatment on in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos, and their pregnancy and delivery rate after embryo transfer into recipient. In experiment 1, to optimize the flavonoid concentration, parthenogenetic day 2 (≥ 2-cell) embryos were cultured in 0 (control), 1, 10 and 20 μM flavonoid for 6 days. In the results, in vitro development rate was the highest in 10 μM flavonoid group (57.1%) among treatment groups (control, 49.5%; 1 μM, 54.2%; 20 μM, 37.5%), and numbers of total and ICM cells were significantly (p<0.05) higher in 10 μM flavonoid group than other groups. We found that 10 μM flavonoid treatment can significantly (p<0.05) decrease the apoptotic index and derive high expression of anti-oxidant, anti-apoptotic, cell growth and development marker genes such as Mn-SOD, Survivin, Bax inhibitor, Glut-5, In-tau, compared to control group. In experiment 2, to produce the cloned Jeju Black Cattle, beef quality index grade 1 bull somatic cells were transferred into enucleated bovine MII oocytes and reconstructed embryos were cultured in 10 μM flavonoid added medium. When the in vitro produced day 7 or 8 SCNT blastocysts were transferred into a number of recipients, 10 μM flavonoid treatment group presented higher pregnancy rate (10.2%, 6/59) than control group (5.9%, 2/34). Total three cloned Jeju Black calves were born. Also, two cloned calves in 10 μM flavonoid group were born and both were all healthy at present, while the one cloned calf born in control group was dead one month after birth. In addition, when the result of short tandem repeat marker analysis of each cloned calf was investigated, microsatellite loci of 11 numbers matched genotype between donor cell and cloned calf tissue. These results demonstrated that the flavonoid addition in culture medium may have beneficial effects on in vitro and in vivo developmental capacity of SCNT embryos and pregnancy rate.

목차

ABSTRACT
 서론
 재료 및 방법
  소 난자의 회수 및 체외성숙
  단위 발생 유도와 체외 배양
  이중 형광 염색
  세포 사멸 분셕
  Real time-PCR을 이용한 유전자 발현 분석
  소 체세포 핵이식 배아의 생산 및 체외 배양
  핵이식 배아의 이식 및 체세포 복제 소 생산
  친자 감별 마커를 이용한 복제유무 검정
 결과
  플라보노이드 처리가 소 단위발생란의 체외발달에 미치는 영향
  플라보노이드 처리가 소 단위발생 배아의 세포사멸에 미치는 영향
  플라보노이드 처리가 소 단위발생 배아의 체 유전자 발현에 미치는 영향
  소 체세포 복제 배아의 체외발달에 플라보노이드 처리가 미치는 영향
  플라보노이드 처리 환경으로서 생산된 체세포 복제 배아의 임신 결과
  친자 감별 유전자 분석에 의한 복제 소의 검정
 고찰
 인용문헌

키워드

저자

• 김은영 [ Eun Young Kim | 미래생명공학연구소, 제주대학교 줄기세포연구소연구센터 ]
• 김연옥 [ Yeon Ok Kim | 미래생명공학연구소 ]
• 김재연 [ Jae Youn Kim | 미래생명공학연구소, 제주대학교 줄기세포연구소연구센터 ]
• 박민지 [ Min Jee Park | 미래생명공학연구소, 제주대학교 줄기세포연구소연구센터, 제주대학교 ]
• 박효영 [ Hyo Young Park | 미래생명공학연구소, 제주대학교 줄기세포연구소연구센터 ]
• 한영준 [ Young Joon Han | 제주대학교 줄기세포연구소연구센터, 제주대학교 ]
• 문성호 [ Seong Ho Mun | 제주대학교 줄기세포연구소연구센터 ]
• 오창언 [ Chang Eon Oh | 제주대학교 줄기세포연구소연구센터, 제주대학교 ]
• 김영훈 [ Young Hoon Kim | 제주대학교 줄기세포연구소연구센터, 제주대학교 ]
• 이성수 [ Sung Soo Lee | 제주특별자치도 축산진흥원 ]
• 고문석 [ Moon Suck Ko | 국립축산과학원 난자축산시험장 ]
• 박세필 [ Se Pill Park | 제주대학교 줄기세포연구소연구센터, 제주대학교 줄기세포연구소연구센터, 제주대학교 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636