Earticle

초록

영어

Pancreatic islet transplantation is now been used as a new strategy to treat Type I diabetes. One of the major obstacles in islet transplantation is the difficulty to preserve islets cells, which result in loss of cell viability during isolation and culture. Gene transfer into islet cell clusters could
improve islet functions and viability via enhancing anti-apoptotic effect and insulin sensitivity to glucose. We investigated whether the genetically modified ICC can successfully be used for transplantation. Rat islets were dispersed and treated with SP-Ex-4 lentivirus for 5 h. After reaggregation, islet 500 and 1000 IEQ of ICC was implanted into diabetic Balb/c nude mice. The graft function over a 30-days period was observed with regard to glucose and insulin level, glucose tolerance tests, and graft insulin content. Our study reveals that reaggregated ICC have better viability rate and gene transduction efficiency than intact islets. High gene transduction of dispersed islets is attributable to the greater number of cells becoming exposed to the lentivirus. SP-Ex-4 transduced ICC enhanced the responsiveness to glucose, thus an effective reverse
hyperglycaemia can be achieved. Therefore, Exendin-4 transduction into ICC is needed for successful islet transplantation.

키워드

저자

• Simmyung YOOK [ College of Pharmacy, Seoul National University. ]
• Jee-Heon JEONG [ College of Pharmacy, Seoul National University. ]
• Yoonsuk JUNG [ College of Pharmacy, Seoul National University. ]
• Youngro BYUN [ College of Pharmacy, Seoul National University. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576