The anti-aging and whitening effects of Aloe extract were studied using proteome analysis. Aloe extracts were prepared by hot water, ethanol, and supercritical fluid extraction. supercritical fluid extraction was performed at pressure of 200~350 bar and temperature of 40~70℃. The effect of extract was measured antioxidant activity [DPPH (1,1-diphenyl- 2-picryl hydrazyl) radical scavenging assay] and skin whitening activity (Tyrosinase inhibition assay). Use proteome analysis (2D-PAGE), expression level changes of the main proteins related to skin aging inhibition and skin whitening by Aloe extracts were observed. The human fibroblasts and mouse B16F1 melanoma cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with or without extracts. Isoelectric focusing (IEF) was carried out using commercial immobiline dry strips 24cm. SDS-PAGE was performed using 25 x 21cm, 10~12.5% polyacrylamide gel. Gels were stained with silver nitrate. Following proteome analysis could explain the mechanism and expression pattern of skin aging and whitening related proteins.
키워드
skin whiteningskin agingextractionproteome
저자
Eun Hyun KIM [ Dept. of Applied Biotechnology, Ajou University, Suwon. ]
Hye In JANG [ R&D center Cosmetic Research Team, Univera, Seoul. ]
Sang Yo BYUN [ Dept. of Applied Biotechnology, Ajou University, Suwon. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동