Earticle

초록

영어

Isoflavones, a family of the major groups of phytoestrogens, are known to have anticarcinogenic, antioxidant and photoprotective effects. Although they have beneficial bioactivities for human health, low bioavailability and poor solubility have been problems in applications. To address this problems, we first have optimized large-scale conditions for enzymatic bio-conversion
of crude isoflavone glycosides(≤20%) from soybean into aglycones by using a β-glucosidase. Further purification processes enabled us to obtain more than 90% purity of isoflavone aglycones analyzed by HPLC. The purified isoflavone showed to a have strong anti-inflammation activity. UVB irradiation of HaCaT cells induced tumor necrotic factor-α (TNF-α)production by 3
folds but the treatment of the purified isoflavone reduced TNF-α production back to the normal level. In order to make derivatives of isoflavone, which might have novel bioactivities with better solubility, acylation reactions of the purified isoflavone were first performed and the resulting isoflavone derivatives were purified for further analyses. Especially, mono-palmitoyl isoflavone not only reduced TNF-α production at much lower concentration than that of isoflavone aglycone, but showed increased solubility by 10 folds compared with isoflavone aglycone. Our results show that mono-palmitoyl isoflavone can potentially enhance bioavailability of isoflavone due to increased solubility and bioactivity.

키워드

저자

• Kyeong-hwan HWANG [ Dongguk University. ]
• Dong-hyun KIM [ Dongguk University. ]
• Hyun-kyung CHOI [ Dongguk University. ]
• Eun-ok LEE [ LCS Biotech. ]
• Chang Seo PARK [ Dongguk University, LCS Biotech. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576