SunA YOON, Ji-Young KIM, Dung Hoang NGUYEN, Jimin UM, Hyang-Bok LEE, Jihyuk YU, Choul-Gyun LEE, Eun-Ki KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A119851
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원문정보
초록
영어
Melanin is a pigment that plays an important role in providing coloration and protecting human skin from the harmful effects of UV light radiation. However, the overproduction and accumulation of melanin in the skin could lead to a serious skin disorders. To treat hyperpigmentating diseases such as such as melasma and lentigines, a number of melanogenesis inhibitors have been screened for their effectiveness in reducing melanin but none are completely satisfactory. M uch effort has been paid to develop new therapeutic agents against pigmentation abnormalities, especially using novel biologically active compounds from natural plants. To develop new and safe skin whitening agents from micro algae, several species of Chlorella have been evaluated. From our screening results, Chlorella kessleri culture broth extract can inhibit melanin synthesis of murine B16F10 melanoma cells in a dose dependent manner and exhibited no cytotoxicity up to a concentration of 200 ug/ml. Inhibitory activity on tyrosinase, the key enzyme of melanogenesis, and antioxidant activity were further investigated. This culture broth extract exhibited no inhibitory effects. Based on DPPH assay, this culture medium extract showed minimal antioxidant activity. These results suggest that Chlorella kessleri culture broth extract could be a high potential candidate as a skin depigmenting agent. To isolate the active components in this plant, solvent-solvent partition was performed. The chloroform fraction showed significant inhibition properties on melanin synthesis with low toxicity. For further isolating the effective compound, silica open column chromatography and high performance liquid chromatography were used.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동