Earticle

초록

영어

Melanogenesis is a multistage process involving melanin synthesis, melanin transport and melanosome release. Melanin synthesis is stimulated by various effectors, including α-melanocyte-stimulating hormone (α-MSH), cyclic AMP (cAMP)-elevating agents (forskolin, isobutylmethylxantine, and glycyrrhizin) and ultraviolet light. In this study, the water extract from
mangosteen (garcinia mangostana) leaves was assessed with regard to its effects on melanogenesis in B16F1 melanoma cells line. The mangosteen leaves extract was shown to stimulate melanin synthesis and tyrosinase activity in a dose-dependent manner without any significant effects on cell proliferation. Formation of melanin from cultured B16F1 melanoma induced by mangosteen leaves extract treatment was estimated using spectrophotometer.
In the range of non-cytotoxic concentrations at 7.8x10-4 % ~ 6.2x10-3 % (w/v) mangosteen leaves extract strongly increased melanogenesis. The results obtained clearly shows that mangosteen leaves extract increase melanogenesis almost 2.5 folds compare to the α-MSH control group. In order to clarify the further mechanism of tyrosinase activation by mangosteen leaves extract, the level of tyrosinase expression in B16F1 cells were examine by intracellular
tyrosinase assay and tyrosinase zymography. The result showed that tyrosinase activity was markedly enhanced from mangosteen treated cells. These results suggested that mangosteen leaves extract might be one of the promising candidates in order to treat hypo pigmentation disorder and useful for self tanning cosmetic products.

키워드

저자

• Abdul Hamid MARIANI [ Dongguk University. ]
• Roji Sarmidi MOHAMAD [ Universiti Teknologi Malaysia. ]
• Chang Seo PARK [ Dongguk University. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576