Recently, QD-based fluorescence resonance energy transfer (FRET) probe as a DNA nanosensor has been studied [1]. In this experiment, PDMS-based microfluidic chips using QD-DNA probe have been fabricated for the detection of p53 gene related to genetic disease. The PDMS microfluidic chip with a weir structure has been utilized for trapping microbeads, sample injection to wells, and fluorescence detection. Carboxylated QDs (Em: 605 nm) have been linked to microbeads of polystyrene/divinyl benzene via EDC/NHS crosslinking reaction. After hybridization with target gene, Borate buffer with DNA intercalating dye has been loaded to the micro-channel. Fluorescence spectra have been observed from the hybridized DNA at the channel of weir structure. The FRET efficiency between QD and dye has been measured by photoluminescence spectroscopy. This experiment shows the possibility of rapid detection of DNA via bead-QD complex on microfluidic system.
키워드
bionanosensorsQDsmicrofluidic chip
저자
Jeong Ha YOO [ Department of Chemical and Bioengineering, Kyungwon University, Gyeonggi, 461-701. ]
Jong Sung KIM [ Department of Chemical and Bioengineering, Kyungwon University, Gyeonggi, 461-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동