Recently, various novel biosensors have been widely studied and developed for early diagnostics of various diseases. Dopamine is one of the large of number of neurotransmitters which plays a role as the excitement transmission in a brain cell. Development of neurotransmitter sensor using Fluorescence Resonance Energy Transfer (FRET) is expected to diagnosis or medical treatment for a disease related to the brain such as Alzheimer’s disease, Parkinsonism and High blood pressure in an aging society due to possibility for measurement of low concentration and brain pressure of the brain trouble. FRET is a physical phenomenon between two fluorophores which transfers emission energy from donor to acceptor for excitation. In this paper, FRET between carboxyl quantum dots and Alexa Fluor has been investigated and the effect of FRET efficiency depending on dopamine concentration has been studied. Quantum Dots 525 (QDs 525) as a donor has been immobilized on surface of well plate which is modified functional group, and conjugated protein A with anti-dopamine labeled by Alexa Fluor 546 (AF 546). AF 546 is used as an acceptor. The fluorescent excitation and emission scans for two fluorophores have been obtained using photoluminescence spectroscopy (PL). The FRET efficiency was obtained from intensity ratio of the fluorescent spectra with and without acceptor group. The dependence of FRET efficiency on dopamine concentration shows that dopamine can be detected by monitoring the FRET phenomena between two fluorescent dyes. Neurotransmitter sensor will be able to establish the system which is possible to diagnosis and medical treatment
키워드
FRETBiosensorQuantum Dot
저자
ChangHwan SEO [ Department of Chemical & Bioengineering, Kyungwon University, gyeonggi, 461-701. ]
Jong Sung KIM [ Department of Chemical & Bioengineering, Kyungwon University, gyeonggi, 461-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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