Yun-Ji SHIN, Kyu-Boem HAN, Ju KIM, Hong-Soo DOO, Moon-Sik YANG, Tae-Ho KWON
언어
영어(ENG)
URL
https://www.earticle.net/Article/A119803
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원문정보
초록
영어
Recently, the rice α-amylase 3D promoter system activated under sucrosestarved conditions has emerged as an alternative system for the production of recombinant proteins. However, the influence of N-linked glycan structures related to sugar starvation conditions has not yet been investigated. The N-glycan patterns obtained from glycoproteins grown in growth medium with sucrose (+S) were different from those obtained under sucrose starvation (-S). The mass spectra of +S glycans exhibited eleven N-glycan patterns: MMXF3, GnMX, GnMXF3, GnGnX, AMXF3, GnGnXF3, AGnXF3, (FA)GnXF3, AAXF3, (FA)(FA)X, and (FA)(FA)XF3. The five N-glycans that disappeared under -S conditions were GnMX, GnGnX, AAXF3, (FA)(FA)X, and (FA)(FA)XF3. The N-glycan pattern of hGM-CSF derived from rice suspension cell culture was similar to that of -S condition, and all N-glycans contained plant-specific β1,2-xyolse, core α1,3-fucose, and some Lewis a epitopes. High-mannose type N-glycans were not detected under either condition (-S or +S) among the secreted glycoproteins. (This Study was supported by Technology Development Program of MIFAFF, and also by a grant from the Ministry of Knowledge Economy)
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동