Adhesion of cells on surface is a basic and important concept in the fields of neural cell culture and morphogenesis of the nervous system. An appropriate material for cell adhesion generally leads to improved differentiation of neuronal cells. Here, we investigated the potential use of a recombinant mussel adhesive protein (MAP), conjugated with laminin derived mimetic peptide as a cell-adhesion material, cost-effective and high-level production in Escherichia coli. Extracellular matrix (ECM) affect process outgrowth, branching of neurites, and profile of neural cells. MAPTrixTM ECM-laminin coatings support cell with the similar diverse morphology compare with other ECMs. ECMs affect adhesion, MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings have greater adhesion compare with poly-l-lysine. ECMs affect expression and growth of axons and dendrites. Greatest number of dendrites and axons are seen on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings. ECMs affect polymerization of actin filaments. High levels of F-actin are produced on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin coatings. ECMs affect expression of the glutamate neurotransmitter receptor. The highest levels of GluR1 are found on MAPTrixTM ECM-laminin, InvitrogenTM human fibronectin and GibcoTM mouse laminin. Therefore, the MAPTrixTM ECM-laminin is suitable for use as a cell-adhesion material in neural cell culture.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동