Earticle

초록

영어

A microorganism isolated from seawater, which utilized rice bran as a substrate and produced carboxymethylcellulase (CMCase), was identified as Bacillus atrophaeus and designated B. atrophaeus LBH-18. Rice bran and peptone were found to be the best combination of carbon and
nitrogen sources for cell growth of B. atrophaeus LBH-18 and the production of CMCase. Based on intuitive analysis and statistical calculations using data from an L25 (53) orthogonal array experiment with four factors and five level of each factor, the optimal concentrations of rice bran and peptone for cell growth of B. atropphaeus LBH-18 were 75.0 and 10.0 g/L, respectively, whereas those for the production of CMCase were 50.0 and 5.0 g/L, respectively. The optimal pH of the medium for cell growth were 6.0, whereas those for the production of CMCa se were 7.0. According to R values calculated from results of an orthogonal array experiment, the most important factor for cell growth as well as the production of CMCase by B. atrophaeus LBH-18 was found to be rice bran. The optimal temperature for cell growth for cell growth as well as the production of CMCase was 30℃.

키워드

저자

• Eun-Jung LEE [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]
• Hye-Jin KIM [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]
• Sang-Un LEE [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]
• Wa GAO [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]
• You-Jung LEE [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]
• Jin-Woo LEE [ Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714. ]

참고문헌

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576