Earticle

초록

영어

Human basophilic KU812F cells play an important role in allergic reactions. In the present study, the effect of solvent fractions from Ishige okamurae on allergic response was examined by measuring histamine release, cytokine production, and FcεRI expression in human basophilic
KU812F cells. Among solvent fractions from I. okamurae, more than 70% of the degranulation of KU812F cells was inhibited by n-hexane and EtOAc (Ethyl acetate) fractions. In addition, n-hexane and EtOAc fractions inhibited the increased IL-4 and IL-13 production and expression. RT-PCR and western blot analysis revealed that n-hexane and EtOAc fractions reduced the regulation of FcεRI α, β and γ chains. Therefore, I. okamurae may exert its anti-allergic activity through negative-regulation of FcεRI expression, inhibiting IL-4 and IL-13 productions and expressions, and decreasing histamine release. Furthermore, we isolated and quantified the polyunsaturated fatty acids from EtOAc fraction with the highest anti-allergy activity as candidate components for anti-allergic activity. These results suggest that I. okamurae exert potent anti-allergic activity, and it could be effectively used in production of anti-allergic pharmaceuticals and cosmaceuticals.

키워드

저자

• Thanh-Sang VO [ Department of Chemistry, Pukyong National University, Busan 608-737. ]
• Jung-Ae KIM [ Department of Chemistry, Pukyong National University, Busan 608-737. ]
• Sang-Hoon LEE [ Marine Bioprocess Research Center, Pukyong National University, Busan 608-737. ]
• Chang-Suk KONG [ Marine Bioprocess Research Center, Pukyong National University, Busan 608-737. ]
• Se-Kwon KIM [ Marine Bioprocess Research Center, Pukyong National University, Busan 608-737. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576