Earticle

초록

영어

A strain of Mannheimia succiniciproducens, a gram-negative rumen bacterium producing succinic acid, was adapted to environment of high-concentration of succinic acid and showed increased acid tolerance. Responses of the adapted M. succiniciproducens strain to succinic acid were studied based on transcriptome analysis, by investigating genetic expression levels of cells after 30 minute and 300 minute of succinic acid shock. During the investigations, gene expression levels of the adapted M. succiniciproducens strain were compared with a parent strain and the expression patterns after acid shock are classified refer to function of genes. Genes related to chaperones, Energy production, metabolism of Amino acid and inorganic ion are increased metabolism generally increase their expression levels. Expression levels of genes for carbohydrate transport and metabolism are decreased in both strains. There are significantly different fold-changes in gene expression levels between both strains in data after 300 minute of succinic acid shock though only small differences were exhibited in 30 minute after data. Standing on the transcriptome data, specific genes of M. succiniciproducens were selected as targets of genetic manipulations and it would contribute to improvement of succinic acid tolerance. [This work was supported by the Genome-Based Integrated Bioprocess Development Project of the Ministry of Education, Science and Technology (MEST). Further supports by the
LG Chem Chair Professorship, Microsoft, IBM SUR, WCU (World Class University) program through the National Research Foundation of Korea funded by MEST (R32-2008-000-10142-0) are appreciated.]

키워드

저자

• Jongho YI [ Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea. ]
• Jong Hwan BAEK [ Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea, Current address: Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. ]
• Sang Yup LEE [ Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea, Department of Bio and Brain Engineering and and Bioinformatics Research Center, KAIST, Daejeon 305-701, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576