Earticle

초록

영어

Despite the promising potential of cell-free protein synthesis, the high cost of the required reagents has plagued its practical application. In particular, most of the reagent cost is attributed to the expenses for conventional energy sources such as phophsoenol pyruvate (PEP) and
creatine phopshate (CP). In this study, for the preparative expression of recombinant proteins at a reduced energy cost, we attempted to use the glycolytic intermediates to drive the cell-free protein synthesis in a continuous-exchange cell-free protein synthesis (CECF) reaction. Among
the glycolytic intermediates examined, use of fructose 1,6-bisphosphate (FBP) gave the highest yield of protein synthesis, producing approximately 7 mg/ml of a chloramphenicol acetyl transferase (CAT) protein. We also found that protein synthesis in the CECF reaction was able to be directed by PCR-amplified linear DNA templates by employing the measures to stabilize mRNA in the reaction mixture.

키워드

저자

• Ho-Cheol KIM [ Dep. of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764. ]
• Kyung-Ho LEE [ Dep. of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764. ]
• Yong-Chan KWON [ Dep. of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764. ]
• Ha-Neul KIM [ Dep. of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764. ]
• In-Seok OH [ Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Dong-Myung KIM [ Dep. of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576